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Electro-Acupuncture Protects Diabetic Nephropathy-Induced Inflammation Through Suppression of NLRP3 Inflammasome in Renal Macrophage Isolation

Author(s):

Jieying Zhang, Xiao Yang, Xinhuan Zhang, Dan Lu and Rui Guo*   Pages 1 - 9 ( 9 )

Abstract:


Background: With the increasing prevalence of diabetes in recent years, diabetic nephropathy (DN) has also become a dangerous disease that greatly endangers human health. The study was designed to determine the mechanism and effect of Electro-acupuncture (EA) in alleviating DN-induced inflammation.

Methods: Mice were intraperitoneally injected with STZ (streptozotocin, 50 mg/kg, S0130, Sigma, St. Louis, MO, USA) for five consecutive days. After 12 weeks after induction, blood glucose levels were measured (>300 mg/dL).

Results: The serum levels of IL-1β and IL-6 were decreased in EA-treated DN mice, and EA protected renal injury. EA could suppress HMGB1/NLRP3/NF-κB pathway. HMGB1 inhibition increased the anti-inflammatory effects of EA on DN by suppressing NLRP3/NF-κB pathway. The activation of HMGB1 attenuated the anti-inflammatory effects of EA on DN by inducing NLRP3/NF-κB pathway.

Conclusions: These results suggested that EA protected DN-induced inflammation through suppression of NLRP3 inflammasome.

Keywords:

Electro-acupuncture, diabetic nephropathy, inflammasome, HMGB1

Affiliation:

Department of Prevention and health care, First Teaching Hospital of Tianjin University of TCM, Tianjin 300381, Department of Prevention and health care, First Teaching Hospital of Tianjin University of TCM, Tianjin 300381, Department of Prevention and health care, First Teaching Hospital of Tianjin University of TCM, Tianjin 300381, Department of pain management, Tianjin first center hospital, Tianjin 300381, Department of Accupuncture, Zibo integrated traditional Chinese and Western Medicine Hospital, Tianjin 300381



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