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Investigation of ACE rs4646994, MTHFR rs1801133 and VDR rs2228570 Genotypes in Jordanian Patients with Fibromyalgia Syndrome

Author(s):

Raida Khalil, Wajdy J. Al-Awaida, Hamzeh J. Al-Ameer*, Yazun Jarrar, Amer Imraish, Omar Al bawareed, Rand Qawadri, Farah Al Madhoon and Loiy Obeidat   Pages 1 - 9 ( 9 )

Abstract:


Background:: Fibromyalgia syndrome (FMS) is a chronic disease characterized by widespread body pain, weakness in certain parts of the body (critical points), low pain tolerance, sleep disturbances, and fatigue. This syndrome is considered rare in Jordan.

Objectives: The research aimed to find out the association of the angiotensin converting enzyme, methylenetetrahydrofolate reductase, and vitamin D receptor (ACE, MHFTR, and VDR, respectively) genotypes with FMS among Jordanian patients.

Methods: This work included 22 FM patients and 22 healthy individuals of Jordanian Arabic origin. The ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 genotypes were determined using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism.

Results: No associations between ACE rs4646994, MTHFR rs1801133, and VDR rs2228570 with the vulnerability of a person for the development of FMS were found. However, we found an association between the ACE rs4646994 genotype and restless leg among FM patients.

Conclusion: Based on the result from this study, it appears that the ACE rs4646994 genotype is associated with restless leg among FMS patients of Jordanian origin. Further clinical investigations with larger sample sizes are required to confirm these findings and understand the molecular mechanism of ACE rs4646994 genetic variant in the restless leg syndrome among FM patients.

Keywords:

Jordan, Fibromyalgia syndrome, ACE rs4646994, MTHFR rs1801133, VDR rs2228570, Jordanian patients.

Affiliation:

Department of Biotechnology and Genetic Engineering, Faculty of Science, Philadelphia University, Amman, Department of Biology and Biotechnology, American University of Madaba, Department of Biology and Biotechnology, American University of Madaba, Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Faculty of Science, Department of Biology, The University of Jordan, Amman 11942, Peoples` Friendship University of Russia (RUDN University), Department of Normal Physiology, Department of Biotechnology and Genetic Engineering, Faculty of Science, Philadelphia University, Amman, Department of Biotechnology and Genetic Engineering, Faculty of Science, Philadelphia University, Amman, Department of Biotechnology and Genetic Engineering, Faculty of Science, Philadelphia University, Amman



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