Submit Manuscript  

Article Details


Establishment of a Mouse Asthenospermia Model through Triggering D-Galactose Mediated Oxidative Stress Injury

Author(s):

Nanjun Liu, Qianxing Wang, Lin Li and Jian Lu*   Pages 1 - 7 ( 7 )

Abstract:


Background: Asthenospermia is defined as forward motility of sperm less than 32%.

Aim/Objective: This study aimed to establish mouse model of asthenospermia through triggering D-galactose mediated oxidative stress.

Materials and methods: Total of 40 Kunming male mice were randomly divided into control group, low-dose group (administrating D-galactose at 60 mg/kg), high-dose group (administrating D-galactose at 120 mg/kg) and high-dose+feed addition group (administrating D-galactose at 120 mg/kg together with oral D-galactose). The testicular weight, testicular organ coefficient, sperm viability, sperm concentration and survival rate of tail of epididymis were measured. Oxidative damage of D-galactose to reproductive system of mice was evaluated by measuring superoxide dismutase (SOD) and malondialdehyde (MDA) in testicular homogenate of mice.

Findings: The sperm motility, motility rate, concentration and survival rate of low-dose, high-dose and high-dose+feed addition group were decreased, compared to that in control group. However, there were significant difference between highdose group/high dose+feed group and control group (p<0.05). The forward motile sperm motility rate and total motility rate accorded with critical criteria of asthenospermia. Comparing with the control group, activity of SOD of model group mice significantly decreased, and MDA concentration significantly increased (p<0.05), excepting for low-dose versus control group for SOD activity. This suggests that testicular tissues suffered from oxidative damage.

Conclusions: This study successfully established a mouse asthenospermia model through D-galactose mediated oxidative stress injury. The establishment of asthenospermia model in this study would provide a new promising insight and act as a potential approach for studying asthenospermia in vivo levels.

Keywords:

Asthenospermia, animal model, D-galactose, oxidative stress, sperm viability.

Affiliation:

Department of Cell Biology, Zunyi Medical University, Zunyi 563000, Department of Cell Biology, Zunyi Medical University, Zunyi 563000, Department of Cell Biology, Zunyi Medical University, Zunyi 563000, Department of Cell Biology, Zunyi Medical University, Zunyi 563000



Read Full-Text article