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Tylophora hirsuta (Wall.) Extracts Ameliorate Diabetes Associated with Inflammation in Alloxan-induced Diabetic Rats

[ Vol. 21 , Issue. 6 ]

Author(s):

Faisal Razzaque, Ali Sharif*, Bushra Akhtar, Humaira M. Khan, Muhammad F. Akhtar, Maryam Zaib, Abdullah Muhammad, Kashif Sohail, Irfan Hamid and Naeem Qaisar   Pages 1031 - 1042 ( 12 )

Abstract:


Background: Tylophora hirsuta (Wall) has long been used as traditional medicine for the treatment of diabetes. The current study is designed to evaluate the anti-diabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta.

Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by the Folin-Ciocalteau method. The anti-oxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti-diabetic assay was conducted using alloxan-induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the pro-inflammatory cytokines (TNF-α and IL-6). The polyphenolic composition of the extract was analyzed using an HPLC system.

Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23 μg/mL), and alpha-amylase inhibition (IC50 =352.8 μg/mL). The aqueous extract of Tylophora hirsuta showed remarkable in-vivo anti-diabetic activity. Results were compared with standard drug glibenclamide. Alloxan induced diabetic mediated alterations in liver function enzymes, renal function determinants, and lipid parameters were significantly restored in aqueous extract treated diabetic rats. A significant reduction in pro-inflammatory cytokines (p<0.001) was observed when compared to the control group. HPLC analysis confirms the presence of quercetin, gallic acid, cinnamic acid, and p-coumaric acid.

Conclusion: These results showed that Tylophora hirsuta possesses strong anti-diabetic and anti-inflammatory potentials and justify its folklore use for the management of diabetes.

Keywords:

Tylophora hirsuta, anti-oxidant activity, anti-diabetic activity, flavonoids, alloxan, diabetic rats.

Affiliation:

Faculty of Pharmacy, The University of Lahore, Lahore, Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Jail Road, Lahore, Department of Pharmacy, University of Agriculture Faisalabad, Faisalabad, Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Jail Road, Lahore, Institute of Pharmaceutical Sciences, Ripah International University, Lahore Campus, Lahore, Johar Institute of Professional Studies, Lahore, Faculty of Pharmacy, The University of Lahore, Lahore, Akson College of Health Sciences, Mirpur, Azad Kashmir, Cadson College of Pharmacy, Kharian, College of Pharmacy, University of Sargodha, Sargodha



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