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Evaluation of Hepatoprotective Activity of Clerodendrum paniculatum Leaf on Carbon Tetrachloride-induced Liver Toxicity Model in Swiss Albino Rats and its Characterization by GC-MS

Author(s):

Remya Kopilakkal and Balamurali MM*   Pages 1 - 13 ( 13 )

Abstract:


Aims: To evaluate the hepatoprotective activity of Clerodendrum paniculatum Leaf On Carbon Tetrachloride Induced Swiss albino Rats and its Characterization by GC-MS.

Background: Clerodendrum paniculatum have ethnomedicinal importance as an ailment for disorders like wound, typhoid, jaundice, malaria and anemia.

Objective: To evaluate the antioxidant and hepatoprotective activity of Clerodendrum paniculatum leaves against CCl4induced rat model and identification of its bioactive constituents by GC MS.

Method: Successive solvent extraction was carried out. Total phenolic, flavonoid content and Antioxidant activity by DPPH, NO, and ABTS method has done. Ethyl acetate extract has been selected for hepatoprotective study by CCl4 intoxicated model followed by the measurement of liver function marker enzymes such as SGOT, SGPT, and ALP. Biochemical parameters like Bilirubin and Protein was measured. Histopathologic liver sections were carried out. Bioactive constituents were evaluated by GC-MS.

Result:By DPPH and ABTS method ethyl acetate extract showed IC50 as 70.14±0.92, 2958.24±2.460 respectively. Alcoholic extract showed maximum IC50 (197.22 ±7.16) for Nitric oxide radical scavenging method. Hepatoprotective study reveals that intoxicated animal groups showed elevated levels of enzymes and bilirubin and suppressed the production of protein. The extract pre treatment showed significant decrease in enzymes and increased production of Total protein in a dose dependant manner. Histopathologic studiesalso support the hepatoprotective activity. GC MS analysis revealed the presence of seven major bioactive constituents with ethyl palmitate as the major one.

Conclusion: The results support the proof for the hepatoprotective potential of the CPLE extract with potent antioxidant activity and enhanced liver enzyme level. The observed activity could be due to the presence of bioactive compounds as identified by GC MS analysis.

Keywords:

Antioxidant activity, Clerodendrum paniculatum, DPPH, NO, Reducing power, ABTS, Hepatoprotective activity, CCl4, GC-MS

Affiliation:

Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Chennai campus, Vandalur-Kelambakkam Road, Chennai - 600 127, Tamil Nadu



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