Submit Manuscript  

Article Details


Arg753Gln Polymorphisms in the Toll-Like Receptor 2 Gene are Associated with Cytomegalovirus Infection in Egyptian Bone Marrow Recipients

[ Vol. 20 , Issue. 4 ]

Author(s):

Sobhy Hassab El-Nabi, Samia Sayed, Mohamed A. Abd-Elhafez, Mohamed Elfiky, Ahmed E. Abdel Moneim and Islam El-Garawani*   Pages 619 - 624 ( 6 )

Abstract:


Background: Previous studies have shown that cytomegalovirus (CMV) induced innate immune response via activation of Toll-like receptor 2 (TLR2). The association between CMV among specific single-nucleotide polymorphisms (SNPs) in the TLR2 gene was also investigated.

Objective: This study investigated the relationship between specific SNPs in the TLR2 gene (G>A), TLR2-Arg753Gln (rs5743708), and CMV replication after bone marrow transplantation.

Methods: The TLR2-Arg753Gln SNP was genotyped in 181 patients after bone marrow transplantation: 83 and 98 patients with and without CMV infection, respectively. CMV load was determined in serially collected blood samples using real-time PCR. Genotyping was performed using specific sequence primer PCR (SSP-PCR), and the results were confirmed by restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified fragments for GG (wild type), GA and AA identification.

Results: Roughly, 85% of the patients screened for the presence of the TLR2-Arg753Gln were GG homozygous, and 15% were GA heterozygous; no patients were homozygous for the mutant allele (A). The GA heterozygous allele was more frequent in the CMV-infected group after bone marrow transplantation.

Conclusion: To our knowledge, this is a novel observation that supports the notion that the functional missense mutation (TLR2-Arg753Gln polymorphism) is possibly associated with CMV replication after bone marrow transplantation. This suggests a role for TLR2 in the innate immune response of human CMV infection in Egyptian bone marrow recipients.

Keywords:

Toll-like receptor 2, bone marrow transplantation, cytomegalovirus, single-nucleotide polymorphisms (SNPs), Arg753Gln, rs5743708.

Affiliation:

Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia

Graphical Abstract:



Read Full-Text article