Adejoke Yetunde Onaolapo, Ebenezer Oladimeji Adebisi, Adegbayi Emmanuel Adeleye, Anthony Tope Olofinnade and Olakunle James Onaolapo* Pages 1 - 14 ( 14 )
Background: Metabolic syndrome is a complex pattern of disorders which occur jointly and is associated with increased risk of cardiovascular and cerebrovascular disease. Therefore the need for more-efficient options of treatment has become imperative.
Objective: This study examined the effect of dietary-melatonin in the management of behavioural, metabolic, antioxidant and organ changes due to high-fat/high-sugar (HFHS) diet- induced metabolic syndrome in mice.
Methodology: Mice were randomly-assigned into five groups of ten animals each. Groups were normal control [fed standard diet (SD)], HFHS control, and 3 groups of melatonin incorporated into HFHS at 2.5, 5 and 10 mg/kg of feed. Mice were fed for seven weeks, and body weight was assessed weekly. Open-field behaviours, radial-arm and Y-maze spatial memory were scored at the end of the experimental period. Twenty-four hours after the last behavioural test, blood was taken for estimation of blood glucose levels after an overnight fast. Animals were then euthanised, and blood was taken for estimation of plasma insulin, leptin and adiponectin levels, and serum lipid profile. The liver, kidneys and brain were excised and processed for general histology, while homogenates of the liver and whole brain were used to assess oxidative stress parameters.
Results: Results showed that dietary melatonin (compared to HFHS diet) was associated with a decrease in body weight, food intake and novelty-induced behaviours; and an increase in spatial-working memory scores. A decrease in glucose, insulin, leptin and malondialdehyde levels; and an increase in adiponectin levels and superoxide dismutase activity were also observed. Histomorphological/histomorphometric examination revealed evidence of organ injury with HFHS diet, and varying degrees of amelioration with melatonin- supplemented diet.
Conclusion: In conclusion, dietary melatonin supplementation may have beneficial effects in the management of metabolic syndrome.
Brain, Dysmetabolism, Diet, Neurobehaviour, Melatonergic, Glucose
Department of Anatomy, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Department of Anatomy, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Department of Anatomy, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Clinical Sciences, College of Medicine, Lagos State University, Ikeja,, Pharmacology and Therapeutics,Ladoke Akintola University,Department of Pharmacology, LAUTECH of Technology