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Deltamethrin-Induced Immunotoxicity and its Protection by Quercetin: An Experimental Study

[ Vol. 20 , Issue. 1 ]

Author(s):

Anoop Kumar*, Meenakshi Gupta, Ruchika Sharma and Neelima Sharma*   Pages 67 - 76 ( 10 )

Abstract:


Background: Deltamethrin (DLM) is a type 2 pyrethroid insecticide used in agriculture and home to control pests. However, emerging reports have indicated the immunotoxicity of DLM.

Objective: Thus, in the current investigation, we have checked the immune-protective role of quercetin in DLM-induced immunotoxicity by using in silico and in vitro techniques.

Results: In silico results have shown good interaction of quercetin towards immune cell receptors (T & B cell receptors). The findings of in vitro studies indicated the decrease in oxidative stress which is elevated by DLM in concentration & time-dependent manner. The increased caspases-3 activity was decreased by treatment of quercetin. The apoptosis induced by DLM in thymus and spleen was suppressed only at higher concentration (50μg/ml) of quercetin. Finally, the phenotypic changes due to DLM were restored by quercetin.

Conclusion: Quercetin has strong binding affinity towards CD4, CD8 and CD28, CD45 receptors and protects the thymocytes and splenocytes against DLM-induced apoptotic signaling pathways.

Keywords:

Pyrethroid insecticide, deltamethrin, oxidative stress, apoptosis, phenotyping, quercetin.

Affiliation:

Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, Department of Pharmacology, ISF College of Pharmacology, Moga, Punjab, Department of Biotechnology, Indo-Soviet Friendship College of Professional Studies (ISFCPS), Moga, Punjab, Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand

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