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Dysregulated Interleukin -33/ST2 Pathway Perpetuates Chronic Inflammation in Hashimoto’s Thyroiditis

[ Vol. 19 , Issue. 7 ]

Author(s):

Xuan Wang, Xiaoqing Shao, Xinhao Liu, Qiu Qin, Jian Xu and Jin A. Zhang*   Pages 1012 - 1021 ( 10 )

Abstract:


Objective: Hashimoto’s Thyroiditis (HT) is an autoimmune disease, characterized by chronic inflammation of the thyroid gland with unknown etiologies. Recently, interleukin-33/ST2 (IL- 33/ST2) pathway reveals its participation in the process of several autoimmune diseases. In this study, the role of IL-33/ST2 pathway in the development of HT is investigated.

Methods: The levels of plasma IL-33, sST2 and the frequency of circulating CD4+ST2L+T cells in 30 HT patients and 20 healthy controls were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry respectively. The mRNA expressions of related molecules in IL-33/ST2 pathway in thyroid tissues (12 HT patients and 10 controls) were detected by real-time quantitative PCR (RTqPCR). The protein expressions of IL-33 and ST2 were determined by Western blot and immunohistochemistry staining.

Results: The mRNA expressions of plasma IL-33 and sST2 were elevated in HT patients, with an increased ratio of IL-33/sST2. The number of CD4+ST2L+ T cells in PBMCs of HT group was significantly increased when compared to the control group (CON) by Flow cytometry assay. MRNA Expression of IL-33 and ST2 in thyroid tissue and the level of IL-1β and IL-18 were significantly upregulated in HT patients, while IL-5 was down-regulated in HT patients, compared to CON. The expression of IL-1β and IL-18 were positively correlated with the expression of IL-33. Results of western blot and immunohistochemical staining were consistent with qPCR.

Conclusion: IL-33/ST2 pathway participates in HT via affecting the production of inflammatory cytokines.

Keywords:

Interleukin-33(IL-33), ST2, hashimoto’s thyroiditis (HT), inflammatory cytokines, soluble ST2, biomarker.

Affiliation:

Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, Department of Endocrinology, Jinshan Hospital of Fudan University, Shanghai, Department of Endocrinology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai

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