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Human Leukocyte Antigens (HLA) Genes Association in Type 1 Diabetic Nephropathy

[ Vol. 19 , Issue. 8 ]

Author(s):

Esma Mihoubi, Habiba Amroun, Rachida Raache*, Faiza Bouldjennet, Fethi Meçabih, Malha Azzouz, Aissa Boudiba, Souad Mahgoun, Malika Akachouche, Nawel Salhi, Chafia Touil-Boukoffa, Mohamed C. Abbadi and Nabila Attal   Pages 1157 - 1164 ( 8 )

Abstract:


Background: Diabetic nephropathy is a common worldwide multifactorial disease where involvement of genetic factors is well etablished. The aim of this study was to investigate the HLA genes implication in the development of type 1 diabetic nephropathy.

Methods: We performed a case- control study where one hundred and fifty subjects were examined. Patients were divided in two groups; with and without type 1 diabetic nephropathy. HLA typing was performed using Polymerase Chain Reaction- Sequence Specific Oligonucleotide (PCR- SSO) method. HLA association to clinical phenotype and HLA haplotype analysis was also investigated.

Results: HLA B*51 is increased in patients without type 1 diabetic nephropathy (7.14% vs. 0 %, P <0.05, OR= 0), however no other studied alleles seem to have any effect (all P>0.05). Haplotype analysis also does not reveal any significant association, however, A*02-B*18-DRB1*03-DQA1*05- DQB1*03 haplotype shows a tendency to be associated with the development of diabetic nephropathy (P = 0.05).

Conclusion: These results suggest a protective effect of HLA B*51 allele from type 1 diabetic nephropathy. However, further studies are required in order to clarify its potential implication as a protective marker.

Keywords:

Type 1diabetes, diabetic nephropathy, class I HLA genes, class II HLA genes, alleles, analysis, haplotype analysis.

Affiliation:

Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team University of Science and Technology, Houari Boumediene (USTHB), Algiers, Central laboratory, Parnet Hospital, Algiers, Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team University of Science and Technology, Houari Boumediene (USTHB), Algiers, Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team University of Science and Technology, Houari Boumediene (USTHB), Algiers, Immunology department, Pasteur Institute of Algiers, Diabetology department of Mustapha Pacha Hospital, Algiers, Diabetology department of Mustapha Pacha Hospital, Algiers, Diabetology department of Mohamed Lamine Debbaghine Hospital, Algiers , Immunology department, Pasteur Institute of Algiers, Immunology department, Pasteur Institute of Algiers, Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team University of Science and Technology, Houari Boumediene (USTHB), Algiers, Immunology department, Pasteur Institute of Algiers, Immunology department, Pasteur Institute of Algiers

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