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IL-1β and IL-1Ra Variant Profiles in Turkish Patients with Diabetic Peripheral Neuropathy

[ Vol. 19 , Issue. 2 ]

Author(s):

Ayse Feyda Nursal*, Ahmet Inanir, Aydın Rustemoglu, Suheyla Uzun, Kubra Sahin and Serbulent Yigit   Pages 150 - 158 ( 9 )

Abstract:


Background: Diabetic peripheral neuropathy (DPN) is one of the most common complications of Type 2 diabetes mellitus (T2DM). This study was conducted to investigate the possible association between interleukin-1β (IL-1β) rs16944 /IL-1 receptor antagonist (IL-1Ra) VNTR variants and genetic susceptibility to DPN in a Turkish cohort.

Methods: A total of 200 subjects were enrolled in this study, 98 patients with DPN and 102 cases of age and sex-matched healthy controls. Genotyping was performed for all individuals using PCR-RFLP analysis.

Results: IL-1β rs16944 CC genotype had a 3.20-fold increased risk for DPN (p=0.0003, OR=3.20, 95% Cl:1.72-5.96). IL-1β rs16944 CT genotype was higher in healthy control than patients (p=0.004). IL-1β rs16944 C allele was higher in the patient group compared to controls while T allele was lower in patients than controls (p=0.003). IL-1Ra VNTR a1/a1 and a2/a2 genotypes were lower in DPN patients while a1/a2 genotype was higher in patients (p=0.045). The patients carrying a1/T haplotype had decreased risk of DPN than control groups (p=0.004). The patients carrying a2/a2 genotype had lower HDL level (p=0.039). The subjects carrying a2/a2 genotype had higher total cholesterol level while the subjects carrying a1/a2 genotype had lower total cholesterol (p=0.026 and p=0.037, respectively). IL-1Ra a1 allele was associated with higher HDL level (p=0.041).

Conclusion: Findings of this study indicated that the IL-1β rs16944 and IL-1Ra VNTR variants are probably to be associated with susceptibility DPN risk in a Turkish cohort.

Keywords:

Type 2 diabetes mellitus, diabetic peripheral neuropathy, interleukin-1β, interleukin-1Ra, IL-1Ra VNTR variants, DPN risk.

Affiliation:

Department of Medical Genetics, Faculty of Medicine, Hitit University, Corum, Turkey; Department of Medical Genetics, Faculty of Medicine, Giresun University, Giresun, Academic Pain-Hernia Center, Samsun, Department of Medical Biology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Department of Internal Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Department of Medical Biology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Department of Medical Biology, Faculty of Medicine, Gaziosmanpasa University, Tokat

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