Chandan K. Jha, Rashid Mir *, Imadeldin Elfaki, Naina Khullar, Suriya Rehman, Jamsheed Javid, Shaheena Banu and Sukh Mohinder Singh Chahal Pages 67 - 74 ( 8 )
Aim: Studies have evaluated the association of miRNA-423 C>A genotyping with the susceptibility to various diseases such cancers, atherosclerosis and inflammatory bowel disease but the results were contradictory. However, no studies have reported the association between miRNA-423 rs6505162 C>A polymorphism and susceptibility of coronary artery disease. MicroRNAs regulate expression of multiple genes involved in atherogenesis. Therefore, we investigated the association of microRNA-423C>T gene variations with susceptibility to coronary artery disease.
Methodology: This study was conducted on 100 coronary artery disease patients and 117 matched healthy controls. The genotyping of the microRNA-423 rs6505162C>A was performed by using Amplification refractory mutation system PCR method (ARMS-PCR).
Results: A significant difference was observed in the genotype distribution among the coronary artery disease cases and sex-matched healthy controls (P=0.048). The frequencies of all three genotypes CC, CA, AA reported in the patient’s samples were 55%, 41% and 4% and in the healthy controls samples were 55%, 41% and 4% respectively. Our findings showed that the microRNA-423 C>A variant was associated with an increased risk of coronary artery disease in codominant model (OR = 1.96, 95 % CI, 1.12-3.42; RR 1.35(1.05-1.75, p=0.017) of microRNA-423CA genotype and significant association in dominant model (OR 1.97, 95% CI (1.14-3.39), (CA+AA vs CC) and non-significant association for recessive model (OR=1.42, 95%CI=0.42-4.83, P=0.56, AA vs CC+CA).While, the A allele significantly increased the risk of coronary artery disease (OR =1.56, 95 % CI, 1.03-2.37; p=0.035) compared to C allele. Therefore, it was observed that more than 1.96, 1.97 and 1.56 fold increased risk of developing coronary artery disease.
Conclusion: Our findings indicated that microRNA-423 CA genotype and A allele are associated with an increased susceptibility to Coronary artery disease.
MicroRNA-423, coronary artery disease (CAD), congenital heart disease (CHD), cerebrovascular disease, Amplification refractory mutation system PCR method (ARMS-PCR), polymorphism, genotype.
Department of Human Genetics Punjabi University, Punjab, Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Department of Biochemistry, Faculty of Science, University of Tabuk, Department of Zoology, Mata Guri College, Institute of Research and Medical Consultation, Imam Abdulrahman Bin Faisal University,Dammam, Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Sri Jayadeva Institute of Cardiovascular science & Research, Bangalore, Department of Human Genetics Punjabi University, Punjab